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DMEM(H)(含雙抗，不含丙酮酸鈉) 培養(yǎng)基
貨號(hào)：12100-046
規(guī)格：10*1L
英文名稱(chēng)：DMEM, Powder, High Glucose
儲(chǔ)存條件：2-8℃
單位：瓶

描述

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

在中國(guó)境內(nèi), 本產(chǎn)品僅限于非臨床科研用途, 產(chǎn)品信息以中文為準(zhǔn)。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

內(nèi)容及儲(chǔ)存

儲(chǔ)存條件：2-8° C
運(yùn)輸條件：環(huán)境溫度
保存期：自生產(chǎn)日期起36個(gè)月

DMEM(H)(含雙抗，不含丙酮酸鈉) 培養(yǎng)基

 參考文獻(xiàn)：

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《Selective anticancer activity of the novel steroidal dihydropyridine spirooxindoles against human esophageal EC109 cells》 作者:Xiaoli Shi,Tiantian Qin,Weihua Liu,Xueyan Zhang,Leilei Li,Junfeng Huo,Kairui Zhou,Dongxiao Yang,Yanling Zhang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:
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《Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map》 作者:Jiayu Gao, Xiao Yang, Jiangxia Hu and Weiping Yin 期刊:International Journal of Pharmacology 影響因子:0.754 PMID:
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《Emodin Attenuates Lipopolysaccharide-Induced Injury via Down-Regulation of miR-223 in H9c2 Cells》 作者:Yuping Yang, Zijun Jiang, Dong Zhuge 期刊:International Heart Journal 影響因子:2.226 PMID:30745529
《miR?9 depletion suppresses the proliferation of osteosarcoma cells by targeting p16》 作者:Song Gao, Jianchao Wang, Shujian Tian, Jianping Luo 期刊:International Journal of Oncology 影響因子:3.571 PMID:31081054
《Long Non-Coding RNA NIFK-AS1 Augments the Oncogenicity of Hepatocellular Carcinoma by Regulating miR-497/BIRC5 Axis》 作者:Yajing Feng,Lu Sun,Yajuan Feng,Zhixian Guo 期刊:SSRN 影響因子: PMID:
《Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress》 作者:Lixin Huang,Min Huang,Mingyue Shen,Pingwei Wen,Ting Wu,Yuzhen Hong,Qiang Yu,Yi Chen,Jianhua Xie 期刊:International Journal of Biological Macromolecules 影響因子:4.784 PMID:31254574
《miR-185 silencing promotes the progression of atherosclerosis via targeting stromal interaction molecule 1》 作者:Ming Fang, Yanfei Li, Yingbiao Wu, Zhongping Ning, Xuejun Wang & Xinming Li 期刊:Cell Cycle 影響因子:3.259 PMID:30784343
《Insulin-like growth factor 2 mRNA binding protein 2 promotes aerobic glycolysis and cell proliferation in pancreatic ductal adenocarcinoma via stabilizing GLUT1 mRNA》 作者:Shan Huang , Zheng Wu , Yunyun Cheng , Wenzhen Wei  Linlin Hao 期刊:Acta Biochimica et Biophysica Sinica 影響因子:2.502 PMID:31089713
《Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression》 作者:Longfei Xiao ， Liangli Song，  Junjie Hu ， Quanwei Zhang ， Yuting Jiang ， Hongwei Duan ， Yong Zhang， Xingxu Zhao ， Yongli Hua 期刊:Journal of Cellular Physiology 影響因子:4.522 PMID:30671954
《Biological characteristics of heat shock protein 90 in human liver cancer cells》 作者:Li Qin, Haixin Huang, Jian Huang, Guodong Wang, Jinxin Huang, Xintian Wu, Jinzhuan Li, Weili Yi, Longjing Liu, and Dongning Huang 期刊:American Journal of Translational Research 影響因子:3.266 PMID:31105855
《Effect of Artesunate on the Expression of ICAM-1 and MMP-9 in Vascular Endothelial Cells under High Glucose Condition》 作者:Pengfei Ge, Tao Jiang, Yao Zong, Xuejiao Yang, Yuna Ma, Yunxiao Wang, Zhe Sun, Ziqun Cao 期刊:Hans Journal of Ophthalmology 影響因子:1.58 PMID: